Ultra High Def™ Blue-AP is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase. Ultra High Def™ Green-AP has been reformulated to increase stability of the working solution while producing a distinct bright blue color. Ultra High Def™ Blue-AP is insoluble in alcohol and xylene substitutes (both aliphatic hydrocarbon and citrus based); therefore sections can be dehydrated in alcohol, cleared in xylene substitute and permanently mounted. However we recommend air drying slides and then permanently mounting. This chromogen substrate system may be used for both automation and manual use.