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AFB Stain Kit

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Stains for mycobacteria (acid fast bacteria) in less than 15 minutes.

This kit is suitable in lieu of and comparable to the Kinyoun’s AFB and Ziehl-Neelsen procedures for staining all mycobacteria, including Mycobacterium tuberculosis. The MasterTech AFB Stain Kit differs from these procedures in that Advanced Carbol Fuchsin is used for bacterial staining instead of a standard Carbol Fuchsin, and Light Green is used as a counterstain instead of Methylene Blue. In comparison to standard Carbol Fuchsins, MasterTech ACF™ requires no heat and much shorter staining times by combining all three fuchsin dyes. Light Green is a much better counterstain than Methylene Blue, as it provides higher contrast against the red stained bacteria.


Stains for mycobacteria (acid fast bacteria) in less than 15 minutes. This kit is suitable in lieu of and comparable to the Kinyoun’s AFB and Ziehl-Neelsen procedures for staining all mycobacteria, including Mycobacterium tuberculosis. The MasterTech AFB Stain Kit differs from these procedures in that Advanced Carbol Fuchsin is used for bacterial staining instead of a standard Carbol Fuchsin, and Light Green is used as a counterstain instead of Methylene Blue. In comparison to standard Carbol Fuchsins, MasterTech ACF™ requires no heat and much shorter staining times by combining all three fuchsin dyes. Light Green is a much better counterstain than Methylene Blue, as it provides higher contrast against the red stained bacteria. 100ml Stain Kit yields between 80 and 100 slides. Pint Stain Kit yields between 320-500 slides. Liter Stain Kit yields 680-1000 slides. Slide Yields assume maximum slides stained per run, maximum runs per use. Actual Slide Yields may vary.

  • KTAFB   100ml Stain Kit yields between 80-100 slides.
  • KTAFBPT Pint Stain Kit yields between 320-500 slides.
  • KTAFBLT Liter Stain Kit yields between 680-1,000 slides.

100ml Kit Consists of: Advanced Carbol Fuchsin 100ml, Light Green Counterstain 100ml, 1% Acid Alcohol Pint.

Pint Kit Consists of: Advanced Carbol Fuchsin Pint, Light Green Counterstain Pint, 1% Acid Alcohol 2 Pints

Liter Kit Consists of: Advanced Carbol Fuchsin Liter, Light Green Counterstain Liter, 1% Acid Alcohol 2 Liters

All kit components are available for purchase individually. Both kits are sealed with a tamper-evident label for quality assurance. All kit components should be stored in a dry environment between 15º C and 30º C. Please review all component labels to ensure correct storage temperature.

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