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Trichrome Stain Kit - Masson's 2000

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Stains for collagen and muscle in less than 30 minutes.

Trichrome stains are commonly used to identify increases in collagenous tissue to assist in the diagnosis of diseases such as liver cirrhosis. This kit includes a 40 minute microwave procedure and the traditional two hour procedure. The Masson’s 2000 Stain Kit is a low hazard alternative to the traditional Masson’s Trichrome Stain Kit which uses Bouin’s Fluid. Instead, a specially formulated Bouin's 2000™ is used, that is free of both formaldehyde and picric acid. Biebrich Scarlet/Acid Fuchsin and Aniline Blue serve as the primary stains, producing red cytoplasm, intercellular fiber, keratin, and muscle, blue collagen and mucus with purple to black nuclei, counterstained by Weigert’s Hematoxylin.


  • KTMTR2   100ml Stain Kit yields between 80-100 slides.
  • KTMTR2PT Pint Stain Kit yields between 320-500 slides.
  • KTMTR2LT Liter Stain Kit yields between 680-1,000 slides.

Stains for collagen and muscle in less than 30 minutes. Trichrome stains are commonly used to identify increases in collagenous tissue to assist in the diagnosis of diseases such as liver cirrhosis. This kit includes a 40 minute microwave procedure and the traditional two hour procedure. The Masson’s 2000 Stain Kit is a low hazard alternative to the traditional Masson’s Trichrome Stain Kit which uses Bouin’s Fluid. Instead, a specially formulated Bouin's 2000™ is used, that is free of both formaldehyde and picric acid. Biebrich Scarlet/Acid Fuchsin and Aniline Blue serve as the primary stains, producing red cytoplasm, intercellular fiber, keratin, and muscle, blue collagen and mucus with purple to black nuclei, counterstained by Weigert’s Hematoxylin. 100ml Stain Kit yields between 80 and 100 slides. Pint Stain Kit yields between 320-500 slides. Liter Stain Kit yields 680-1000 slides. Slide Yields assume maximum slides stained per run, maximum runs per use. Actual Slide Yields may vary.

100ml Kit Consists of: Bouin's 2000â„¢ 100ml, Biebrich Scarlet/Acid Fucshin 100ml, Phosphomolybdic/Phosphotungstic Acid 100ml, Aniline Blue Stain 100ml, 1% Acetic Acid 100ml and Weigert's A & B Hematoxylin 100ml each.

Pint Kit Consists of: Bouin's 2000â„¢ Pint, Biebrich Scarlet/Acid Fucshin Pint, Phosphomolybdic/Phosphotungstic Acid Pint, Aniline Blue Stain Pint, 1% Acetic Acid Pint and Weigert's A & B Hematoxylin Pint each.

Liter Kit Consists of: Bouin's 2000â„¢ Liter, Biebrich Scarlet/Acid Fucshin Liter, Phosphomolybdic/Phosphotungstic Acid Liter, Aniline Blue Stain Liter, 1% Acetic Acid Liter and Weigert's A & B Hematoxylin Liter each.

All kit components are available for purchase individually. Both kits are sealed with a tamper-evident label for quality assurance. All kit components should be stored in a dry environment between 15º C and 30º C. Please review all component labels to ensure correct storage temperature.

American MasterTech recommends using Uterus Control Slides, Trichrome Control Slides, Heart Control Slides and Skeletal Muscle Control Slides.

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